Table 1.
Specific primers used for RT-PCR and qRT-PCR.
| Gene | Primers | cDNA product size | Genomic DNA product size |
|---|---|---|---|
| P5C synthetase 1 (protein ID Q941T1) | fwd CGCAGGATCAATTCGTGAAATCGCA rev GCAATCTGTACCAAGGCATCAGGA |
162 | 291 |
| P5C synthetase 2 (protein ID O04226) | fwd GATTGGGTGCTGAGGTTGGCATAA rev CGACATCCTTGTCACCATTCACCA |
132 | – |
| P5C reductase (protein ID Q8GT01) | fwd ATCGTAAGGCAGGTACTGGTTGAG rev AGCTGAACGGGTGTTTGGCATT |
150 | 377 |
| Ornithine-δ-aminotransferase (protein ID Q10G56) | fwd GAGGGGTTTGCTTAATGCAGTGGA rev CAGCGGAGGCGCTAATCTGATTAT |
142 | 142 |
| Proline dehydrogenase (protein ID Q336U3) | fwd ACGGTCCAGTGGAGCAGATCAT rev AGCATTGCAGCCTTGAACCT |
133 | 261 |
| P5C dehydrogenase (protein ID B9FLB9) | fwd ATGCTTGCAGTGGTCAGAAGTG rev TGGGCCAATGGTCAAGTCTTCA |
134 | 487 |
| Actin-1 (protein ID Q10DV7) | fwd CTGCGGGTATCCATGAGACT rev GCAATGCCAGGGAACATAGT |
118 | 118 |
| Elongation factor (EF) 1α (protein ID O64937) | fwd TCTCTGGGTTTGAGGGTGACAACA rev TTGATCTGGTCAAGAGCCTCAAGC |
94 | 94 |
Sequences were designed using the Primer 3 software (http://bioinfo.ut.ee/primer3-0.4.0/).