Table 2.

Properties of rice P5C dehydrogenase.

Denatured molecular mass (by SDS-PAGE)	58.2 ± 1.3 kDa
Native molecular mass (by gel permeation)	259 ± 14 kDa
Isoelectric point	5.60 ± 0.05
pH optimum	6.72
Temperature optimum	46 ± 1°C
Activation energy	54.6 ± 3.7 kjoules mol−1
V${}_{\text{max }({NAD}^{+})}$	196.9 ± 2.1 nkat (mg protein)−1
V${}_{\text{max }(P5C,{withNAD}^{+}astheco-substrate)}$	207.7 ± 3.3 nkat (mg protein)−1
V${}_{\text{max }({NADP}^{+})}$	49.2 ± 1.7 nkat (mg protein)−1
V${}_{\text{max }(P5C,{withNADP}^{+}astheco-substrate)}$	51.0 ± 1.0 nkat (mg protein)−1
K${}_{\text{cat }({NAD}^{+})}$	12 s−1
K${}_{\text{cat }({NADP}^{+})}$	3 s−1
KM(app) for L-P5C ${}_{(\text{NA}{\text{D}}^{+})}$	358 ± 14 μM
KM(app) for L-P5C ${}_{(\text{NAD}{\text{P}}^{+})}$	265 ± 13μM
KM(app) for NAD+	644 ± 30 μM
KM(app) for NADP+	4695 ± 462 μM