Figure 4. Identification of the spliced form of RmInt1 in FLAG-immunoprecipitate from transfected HeLa cell.

(a) Representation of the PCR products obtained from the spliced intron as a circle or lariat. The circled A is the bulged adenosine residue in domain VI. (b) RT-PCR products were subjected to electrophoresis in a 2% agarose gel. PCR was performed with (+) or without (−) prior reverse transcription (RT), with the immunoprecipitates indicated. The area in which the DNA fragments were isolated is indicated by a square bracket. Other unspecific PCR products likely derived from contaminant genomic DNA was also observed. M: molecular weight marker; the numbers on the right indicate the size of the marker band in base pairs.