Figure 3. Enzymatic assay on the full-length NosA, NocA and their variants:
(A) NosA and its K49A and E101A variants; (B) NosA and its E101D, E101Q, E101K and K49E variants; (C) NosA1-111, NosA1-111 mixed with NosA112-151 at mole ratio 1:1, and NosA112-151; (D) NocA and its K41A and E93A variants. In all cases, the substrate (top) and the product nosiheptide (bottom) were used as controls, highlighted in a dotted red line and green line, respectively.