Figure 6. The enzymatic assay performed on HPLC systems:

(A) from top to bottom, only substrate as a control, NosA_1-120, NosA_1-140 variants, full-length NosA, and the catalytic reaction product nosiheptide used as another control; (B) from top to bottom, only substrate as a control, NosA_1-111 plus Did2, NosA_1-111 plus JmjN from KDM5C, NosA_1-111 plus Vps60, and the catalytic reaction product nosiheptide used as another control. In (A,B), the controls substrate and the product nosiheptide were indicated by pink and green dotted line, respectively.