Figure 3.

A P element insertion results in partial loss-of-function of Dlc90F, and imprecise excision of the P element abolishes 14-kDa LC expression. (A) A Western blot probed with the 14-kDa LC antibody. Ovary extracts were centrifuged to separate insoluble material, and equal total protein was loaded in each lane. LC expression in flies of genotype Df(3R)DG2/F5 is reduced but not eliminated. (B) Ovary extracts were prepared as in A from the P excision line e155/e155, balanced siblings with one copy of Dlc90F (e155/+), and wild-type (+/+). (C) No protein is detected in extracts from whole male or female adult flies homozygous for e155. WT, wild-type; S, supernatant; P, pellet.