Figure 4.

Morphology of mutant testis and spermatid nuclei. Testis morphology was examined using a phase-contrast microscope. Testes were dissected from wild-type (A) and e155/e155 adult males (B) held away from females for 6 d. The base of a testis is located in the center of the coil. Arrow points to seminal vesicle. (C-F) Confocal sections of testes from 1- to 2-d-old adult males stained with fluorescent Oligreen dye to visualize nuclei. (C) Elongated and bundled spermatid nuclei in each cyst are visible near the base of wild-type testis (arrow). (D) In the e155 mutant, nuclei are not properly bundled and seem scattered throughout the length of the testis. (E) Nuclei of e155 mutant spermatids are scattered along sperm tails of several cysts seen in high magnification. Some nuclei have undergone proper elongation (arrow), whereas others have not (arrowhead). Region shown is near base of testis. (F) Scattered nuclei in a single cyst from e155 mutant testis. Nuclei have not successfully elongated and are randomly oriented. Bar (C-F), 10 μm.