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Immunofluorescence of cells expressing GFP fused to the Rnt1p N-terminal 22 amino acids (GFP-Rnt1p-22st), N-terminal 82 amino acids (GFP-Rnt1p-82st), C-terminal 43 amino acids (GFP-ds4), or the last 22 amino acids at the C terminus (GFP-Rnt1p-451ds). Rnt1p is shown in red, Nop1p in green, and DNA in blue. Colocalization of Rnt1p and Nop1p is in yellow and colocalization of Rnt1p with DNA is in purple. Yellow bar, 2 μm.
