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. 2004 Jul;15(7):3015–3030. doi: 10.1091/mbc.E04-03-0183

Table 2.

Plasmid constructs used in this study

Name Description Source
pRS315/GFP GFP under RNT1 promoter control in pRS315 This work
pRS315/GFP/RNT1 RNT1 coding sequence (1-471 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/ΔNT2 RNT1 coding sequence (192-471 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/NT2 RNT1 coding sequence (1-191 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/ds1 RNT1 coding sequence (344-471 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/ds4 RNT1 coding sequence (428-471 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-22st RNT1 coding sequence (1-22 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-82st RNT1 coding sequence (1-82 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-451ds RNT1 coding sequence (451-471 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-K45/I RNT1 coding sequence (1-471 aa) variant with K45I mutation cloned in frame in pRS315/GFP This work
pRS315/GFP/NT2-K45/I RNT1 coding sequence (1-191 aa) variant with K45I mutation cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-463st RNT1 coding sequence (1-463 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/ds1-463st RNT1 coding sequence (344-463 aa) cloned in frame in pRS315/GFP This work
pRS315/GFP/RNT1-D247/R RNT1 coding sequence (1-471 aa) variant with D247R mutation cloned in frame in pRS315/GFP This work
pGBDU-C3 James et al. (1996)
BD/RNT1 RNT1 coding sequence (1-471 aa) cloned in frame in pGBDU-C3 Lamontagne et al. (2000)
BD/RNT1-D247/R RNT1 coding sequence (1-471 aa) variant with D247R mutation cloned in frame in pGBDU-C3 This work
AD/RNT1-463st RNT1 coding sequence (1-463 aa) cloned in frame in pGAD This work
PNS Ueki et al. (1998)
pNS/NLS Ueki et al. (1998)
pNS/RNT1 RNT1 coding sequence (1-471 aa) cloned in frame in pNS This work
pNS/ΔNT2 RNT1 coding sequence (192-471 aa) cloned in frame in pNS This work
pNS/NT2 RNT1 coding sequence (1-191 aa) cloned in frame in pNS This work
pNS/ds1 RNT1 coding sequence (344-471 aa) cloned in frame in pNS This work
pQE31
pQE/RNT1 RNT1 coding sequence (1-471 aa) cloned in frame in pQE31 Lamontagne et al. (2000)
pQE/RNT1-K45/I RNT1 coding sequence (1-471 aa) variant with K45I mutation cloned in frame in pQE31 This work
pQE/RNT1-463st RNT1 coding sequence (1-463 aa) cloned in frame in pQE31 This work
pQE/RNT1-D247/R RNT1 coding sequence (1-471 aa) variant with D247R mutation cloned in frame in pQE31 This work