Table 1.
Plasmids used in this study
| Plasmid | Backbone | Yeast ori | Insert | Source |
|---|---|---|---|---|
| pFL644 | pRT21 | 2μ | ENT5-(E-GFP3) | This study |
| pFL647 | pGEX4-T1 | ENT5 | This study | |
| pFL653 | pGEX4-T1 | ent5ΔENTH | This study | |
| pFL719 | pGEX4-T1 | ENTH domain of Ent5p | This study | |
| pFL703 | pQE15 | VPS27 | This study | |
| p299 | pGEX2TK | FYVE of Hrs | Sankaran et al. (2001) | |
| pGO45 | pRS426 | 2μ | GFP-Cps1 | Odorizzi et al. (1998) |
| pGFP-Phm5 | pRS416 | CEN | GFP-Phm5 | Reggiori and Pelham (2001) |
| pGFP-DPAP B | pRS426 | 2μ | GFP-DPAP B | Odorizzi et al. (1998) |
| pGFP-Ste2 | pRS426 | 2μ | GFP-Ste2 | Odorizzi et al. (1998) |
| pKN32 | 2μ | UBI4 | Nelson and Lemmon (1993) | |
| pEE27-HA | pRS416 | CEN | VPS27-(HA)3 | Shih et al. (2002) |