Figure 1.
IRI-1, a previously uncharacterized protein, interacts with the regulatory domain of the ITR-1. (A) Schematic diagram of ITR-1, showing IP3 binding, regulatory, and transmembrane domains. Bar below indicates the region identified as interacting with IRI-1. (B) Gene structure of iri-1, based on cDNA sequence. Boxes represent exons. Bar below indicates the region identified in a yeast two-hybrid screen as encoding a peptide that interacts with ITR-1. (C) In vitro binding of35S-IRI-1734-867 with a GST-ITR-11590-1951 fusion protein. (D) Amino acid sequence of IRI-1. ITR-1-interacting sequence is underlined (solid line). Overlapping pat 4 and pat 7 consensus sequences are underlined (dotted line). The region showing structural similarity to a region of RanGAP1, and sequence similarity to cytocentrin and RalBP1, is shown in bold.