Protein kinase C alpha distribution in mouse RBCs is light and activity dependent. Immunofluorescent labeling for PKCα of retina sections from (A) light-adapted and dark-adapted WT mice, and (B) light-adapted TRPM1+/+ and TRPM1−/− mice. (C) Western blot showing the relative quantities of PKCα in TRPM1+/+ and TRPM1−/− retinas. The asterisk denotes a background band that serves as a loading control for equal quantities of protein in each lane. (D) Phospho-serine, TRPM1, and mGluR6 immunofluorescence in the OPL of retina sections from WT and PKC-KO littermates. The scale bar in the lower right panel represents 12 μm and applies to (A, B, D).