Table 1.
Effects of Hypotonic Conditions on the Anterior-Posterior (A-P) Length, Equatorial Diameter (ED), A-P to ED ratio, and Volume of Isolated Rabbit Lenses Held in a Stretched Conformation.
| Time (min) | Lens Conformation | Bath Osmolality (mOsM) | A-P Length (mm) | ED (mm) | A-P/ED | Volume (mm3) | Volume Change (mm3) |
|---|---|---|---|---|---|---|---|
| 0 | Relaxed | 290 | 8.02 ± 0.03 | 10.48 ± 0.03 | 0.764 ± 0.007 | 454 ± 49 | - |
|
| |||||||
| 5 | Stretched | 290 | 7.93 ± 0.03* | 10.54 ± 0.03 | 0.751 ± 0.009* | 448 ± 48* | -6 |
| 15 | “ | 200 | 7.99 ± 0.03 | 10.62 ± 0.03 | 0.751 ± 0.013 | 459 ± 47* | +11 |
| 25 | “ | 200 | 8.04 ± 0.03* | 10.64 ± 0.02 | 0.754 ± 0.011 | 461 ± 48* | +2 |
|
| |||||||
| 26 | Relaxed | 200 | 8.10 ± 0.03*,** | 10.61 ± 0.03** | 0.762 ± 0.010 | 469 ± 51** | +8 |
Values are means ± SEM’s from a set of 6 lenses treated identically. The isolated lenses were photographed from the side at the time intervals shown; pixel dimensions of the resulting images were converted into millimeters as described in Kong et al., 2009. Lens volume was calculated as described in Gerometta et al., 2007. Rabbit lenses were stretched in the chamber described in Zamudio et al., 2008.
Value significantly different than its respective antecedent (P< 0.05, as two-tailed paired data).
Value significantly larger than its respective t= 0, control value (P< 0.05, as two-tailed paired data).