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Lysosomal density in LAMP-1/LAMP-2 double-deficient MEFs. (A) The lysosomal density from control and LAMP-1/LAMP-2 (L1/L2-/-) double-deficient cells was determined in 27% Percoll gradients. β-hexosaminidase activity and the activity of endocytosed horseradish peroxidase (HRP, 5-min feeding and 3-h chase) were measured in the fractions. (B) LIMP-2/LGP-85 content of the fractions was estimated by Western blotting.
