Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Jul;15(7):3146–3154. doi: 10.1091/mbc.E04-02-0092

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The American Society for Cell Biology

PMC Copyright notice

Figure 5. — Rab7 dissociates slowly from Sifs. Control or infected HeLa cells were transfected with Rab7-GFP, and mobility was estimated by FRAP. (A and B) Typical experiment where Sifs are shown immediately before (A) and 60 s after photobleaching (B). The position of the Sifs is bracketed by arrows. (C) Representative quantitation of the fluorescence recovery in uninfected cells transfected with wild-type Rab7-GFP (▪) or Rab7-Q67L (○) or in Sifs from Salmonella-infected HeLa cells transfected with wild-type Rab7-GFP (). (D) Summary of t_1/2 and fractional recovery (R) for each condition. Data are means ± SE of 20 cells. Bars, 10 μm.

Inline graphic — Rab7 dissociates slowly from Sifs. Control or infected HeLa cells were transfected with Rab7-GFP, and mobility was estimated by FRAP. (A and B) Typical experiment where Sifs are shown immediately before (A) and 60 s after photobleaching (B). The position of the Sifs is bracketed by arrows. (C) Representative quantitation of the fluorescence recovery in uninfected cells transfected with wild-type Rab7-GFP (▪) or Rab7-Q67L (○) or in Sifs from Salmonella-infected HeLa cells transfected with wild-type Rab7-GFP (). (D) Summary of t_1/2 and fractional recovery (R) for each condition. Data are means ± SE of 20 cells. Bars, 10 μm.