Figure 2.

CIN85 SH3 domains can cluster numerous effectors. (A) Equal amounts of lysates from HEK293T cells transiently transfected with indicated proteins were incubated with GST alone or GST-fusion proteins encoding the single (SH3A, SH3B, and SH3C), double (SH3AB and SH3BC), or triple (SH3ABC) SH3 domains of CIN85. Top, amounts of precipitated proteins. Bottom, levels of used GST constructs stained with Ponceau. (B) FLAG-tagged constructs encoding wild-type CIN85 or its deletion isoforms were transiently expressed together with FLAG-ASAP or myc-Hip1R in HEK293T cells. Lysates were immunoprecipitated (IP) and immunoblotted (IB) with indicated antibodies.