Fig. 1.

Development of the new heptaplex PCR showing the amplification of single and multiple DNA markers. Lane 1: 100 bp DNA Marker (New England Biolabs, NEB, UK) with upper band [200 bp] and lower band [100 bp], Lane 2: PCR negative control [Candida albicans], Lane 3: coagulase-negative staphylococcus marker [cns, 204 bp], Lane 4: bacterial 16S rRNA marker [16S, 174 bp], Lane 5: mecA marker [mecA, 155 bp], Lane 6: staphylococcus genus translation elongation factor marker [tuf, 143 bp], Lane 7: Panton-Valentine leukocidin marker [pvl, 118 bp], Lane 8: Vancomycin resistance marker [vanA, 111 bp], Lane 9: staphylococcal protein A marker [spa, 101 bp], Lane 10: Heptaplex PCR showing all seven markers (cns, 16S, mecA, tuf, pvl, vanA and spa) from top to bottom respectively