Table 1.
Strains and plasmids
| Strain or plasmid | Genotype or description | Reference/source |
|---|---|---|
| C. albicans strains | ||
| SC5314 | prototrophic | Fonzi and Irwin (1993) |
| CAF2-1 | Fonzi and Irwin (1993) | |
| CAF2-1/01α | as CAF2-1, but MTLα/MTLα | This work |
| CAF2-1/01α-op | as CAF2-1/01α, but opaque growth form | This work |
| CAI4 | Δura3::imm434/Δura3::imm434 | Fonzi and Irwin (1993) |
| CA18 | ade2::hisG/ade2::hisG Δura3::imm434/Δura3::imm434 | Fonzi and Irwin (1993) |
| C4/c, C4/d | as CAI4, but EFH1/efh1Δ::FRT-SAP2p-FLP-URA3-FRT | This work |
| C4/c4, C4/d6 | as CAI4, but EFH1/efh1Δ::FRT | This work |
| C4/d6-3 | as CAI4, but efh1Δ::FRT/efh1Δ::FRT-SAP2p-FLP-URA3-FRT | This work |
| efh/02α | as C4/d6-3, but MTLα/MTLα | This work |
| efh/02α-op | as efh/02α, but opaque growth form | This work |
| C4/d63-1, C4/d63-3 | as CAI4, but efh1Δ::FRT/efh1Δ::FRT | This work |
| JKC19 | as CAI4, but cph1Δ::hisG/cph1Δ::hisG-URA3-hisG | Liu et al. (1994) |
| HLC52 | as CAI4, but efg1Δ::hisG/efg1Δ::hisG-URA3-hisG | Lo et al. (1997) |
| Bca09-4 | as CAI4, but efg1Δ::hisG/efg1Δ::hisG-URA3-hisG | Braun and Johnson (2000) |
| HLC67 | as CAI4, but efg1Δ::hisG/efg1Δ::hisG | Lo et al. (1997) |
| HLC67[pDB35]a | HLC67 carrying pDB35, made MTLa/MTLa | This work |
| HLC74 | as CAI4, but efg1Δ::hisG/efg1Δ::hisG (EFG1) | Lo et al. (1997) |
| HLC54 | as HLC52, but cph1Δ::hisG/cph1Δ::hisG | Lo et al. (1997) |
| H/1.22, H/1.26 | as HLC67, but efh1Δ::hisG/efh1Δ::hisG-URA3-hisG | This work |
| H/1.22-1, H/1.26-1 | as HLC67, but efh1Δ::hisG/efh1Δ::hisG | This work |
| CRC101-103 | as CAI8, but containing ADH1-lacZ::ADE2 | Russell and Brown, unpublished data |
| CRC104-106 | as CAI8, but containing lexA-ADH1-lacZ::ADE2 | Russell and Brown, unpublished data |
| Red3/6 | ade2/ade2, phenotypic switching strain | Srikantha et al. (2000) |
| Transformation vectors | ||
| pRC18 | URA3-marked CARS2-vector | Stoldt et al. (1997) |
| pBI-1 | PCK1-promoter in URA3-marked CARS2-vector | Stoldt et al. (1997) |
| pAPE(2)/ADE | PCK1-promoter-EFG1 fusion in CARS2 ADE2-vector | Sonneborn et al. (1999b) |
| pRC2312P-H | PCK1-promoter-EFG1 fusion in pBI-1 | Stoldt et al. (1997) |
| pDB35 | PCK1-promoter-EFH1 fusion in pBI-1 | This work |
| pBT-4 | CARS2 ADE2-vector | Sonneborn et al. (1999b) |
| pBT-44 | PCK1-promoter in CARS2 ADE2-vector | This work |
| pBT-145 | PCK1-promoter-EFH1 fusion in pBT-4 | This work |
| pTD13 | PCK1-promoter-HA-EFH1 fusion in pBI-1 | This work |
| pBT-34 | Major EFG1-promoter-LAC4 fusion in CARS2 URA3-vector | Tebarth et al. (2003) |
| p89a | Major EFG1-promoter-RLUC fusion in URA3-vector | This work |
| pLR6 | EFH1 promoter-RLUC fusion in URA3 vector | This work |
| CIp-LexA | URA3-marked integrating vector containing ACT1p-lexA | Russell and Brown, unpublished data |
| CIp-LexA-Efg1 | as CIp-LexA, but containing ACT1p-lexA-EFG1 | This work |
| CIp-LexA-Efh1 | as CIp-LexA, but containing ACT1p-lexA-EFH1 | This work |
| pGBD-C1 | TRP1 vector with GAL4-BD | James et al. (1996) |
| pGAD-C1 | LEU2 vector with GAL4-AD | James et al. (1996) |
| pDB17 | pGBD-C1 containing EFG1 | This work |
| pDB16 | pGAD-C1 containing EFG1 | This work |
| pDB34 | pGBD-C1 containing EFH1 | This work |
| pDB33 | pGAD-C1 containing EFH1 | This work |
| pSKM69 | pGBD-C1 containing efh1ΔN1 | This work |
| pSKM68 | pGAD-C1 containing efh1ΔN1 | This work |
| pCS5 | pGBD-C1 containing efh1ΔN2 | This work |
| pCS6 | pGAD-C1 containing efh1ΔN2 | This work |
| pCS7 | pGBD-C1 containing efh1ΔN3 | This work |
| pCS8 | pGAD-C1 containing efh1ΔN3 | This work |