Table 2.
Regulation of genes encoding metabolic enzymes
| Accession no. |
Fold regulation |
||||
|---|---|---|---|---|---|
| Gene | Description (CandidaDB) | efg1 | efg1-efh1 | efh1 | |
| Glycolysis | |||||
| ENO1 | CA3874 | Enolase I | 0.66 | 0.60 | 0.96 |
| FBA1 | CA5180 | Fructose-bisphosphate aldolase | 0.37 | 0.46 | 0.99 |
| GLK1 | CA0263 | Aldohexose specific glucokinase | 0.40 | 0.54 | 0.80 |
| GPM1 | CA4671 | Phosphoglycerate mutase | 0.40 | 0.56 | 0.99 |
| PFK1 | CA1834 | Phosphofructokinase, alpha subunit | 0.46 | 0.53 | 0.97 |
| PFK2 | CA3112 | Phosphofructokinase, beta subunit | 0.41 | 0.65 | 0.93 |
| PGI1 | CA3559 | Glucose-6-phosphate isomerase | 0.50 | 0.64 | 0.96 |
| TPI1 | CA5950 | Triose phosphate isomerase | 0.44 | 0.58 | 0.82 |
| HXK2.3F | CA0127 | Hexokinase II | 0.36 | 0.49 | 0.76 |
| Reserve | |||||
| carbohydrates | |||||
| TPS2 | CA5066 | Threalose-6-phosphate phosphatase | 0.45 | 0.83 | 0.89 |
| TPS3.3 | CA5505 | Trehalose-phosphate synthase, regulatory subunit | 0.41 | 0.49 | 0.92 |
| IPF8321 | CA2938 | Initiator of glycogen synthesis | 0.41 | 0.53 | 1.01 |
| GSY1 | CA5467 | Glycogen synthase | 0.45 | 0.51 | 1.04 |
| GPH1 | CA5206 | Glycogen phosphorylase | 0.35 | 0.57 | 0.98 |
| RHR2 | CA5788 | DL-glycerol phosphatase | 0.23 | 0.60 | 1.16 |
| Citric acid | |||||
| cycle | |||||
| ACC1 | CA5816 | Acetyl-coenzyme-A carboxylase | 1.5 | 1.42 | 1.08 |
| ACH1 | CA0345 | Acetyl-coenzyme-A hydrolase | 2.3 | 1.82 | 1.10 |
| FUM12.3F | CA4351 | Fumarate hydratase, 3-prime end | 3.1 | 1.60 | 1.10 |
| FUM12.5F | CA4349 | Fumarate hydratase, 5-prime end | 2.1 | 1.57 | 1.05 |
| GDH3 | CA1579 | NADP-glutamate dehydrogenase | 2.5 | 0.79 | 1.07 |
| IPF4401 | CA0903 | Succinate dehydrogenase | 1.7 | 0.99 | 1.24 |
| KGD1 | CA3149 | 2-oxoglutarate dehydrogenase | 2 | 1.22 | 1.15 |
| MDH1 | CA5164 | Mitochondrial malate dehydrogenase precursor | 1.6 | 1.21 | 1.20 |
| SDH12 | CA2470 | Succinate dehydrogenase | 1.5 | 1.08 | 1.09 |
| ACS2 | CA2858 | Acetyl-coenzyme-A synthetase | 1.51 | 1.5 | 1.02 |
Expression of genes in an efg1 mutant (HLC52), an efh1 mutant (C4/d6-3) and an efg1 efh1 mutant (H/1.22) relative to the wild-type CAF2-1. Cells were grown in YPD medium at 30°C prior to transcriptional profiling.