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. 2015 Jun 1;8(6):6181–6191.

Table 2.

FAM labeled primers for GAPDH, NUMB, APP and VEGFA gene alternative splicing quantization

Gene name Primer sequence (5’ to 3’) Amplicon size Primer Starts/Ends
h-gapdh-F FAM-TGAAGGTCGGAGTCAACGGA 225 bp
h-gapdh-R CCTGGAAGATGGTGATGGGAT
h-numb-F FAM-TGCTCCGATGACCAAACCAG 157 bp (PRRS)/301 bp (PRRL) EXON 11
h-numb-R CACCTCTTCTAACCATCGGTC EXON 13
h-app-F FAM-CCTACGAAGAAGCCACAGAG 162 bp/330 bp/387 bp EXON 6
h-app-R GGGCATGTTCATTCTCATCC EXON 9
h-vegfa-F FAM-TGAGCTTCCTACAGCACAAC 105 bp/185 bp/205 bp/338 bp EXON 5
h-vegfa-R TCGATGGTGATGGTGTGGTG EXON 8