Table 2.
Gene name | Primer sequence (5’ to 3’) | Amplicon size | Primer Starts/Ends |
---|---|---|---|
h-gapdh-F | FAM-TGAAGGTCGGAGTCAACGGA | 225 bp | |
h-gapdh-R | CCTGGAAGATGGTGATGGGAT | ||
h-numb-F | FAM-TGCTCCGATGACCAAACCAG | 157 bp (PRRS)/301 bp (PRRL) | EXON 11 |
h-numb-R | CACCTCTTCTAACCATCGGTC | EXON 13 | |
h-app-F | FAM-CCTACGAAGAAGCCACAGAG | 162 bp/330 bp/387 bp | EXON 6 |
h-app-R | GGGCATGTTCATTCTCATCC | EXON 9 | |
h-vegfa-F | FAM-TGAGCTTCCTACAGCACAAC | 105 bp/185 bp/205 bp/338 bp | EXON 5 |
h-vegfa-R | TCGATGGTGATGGTGTGGTG | EXON 8 |