Figure 4.

miR-15a directly targets TNFAIP1 by binding to its 3’-UTR. A. The predicted miR-15a binding site within the TNFAIP1 3’-UTR and a mutated version generated by site directed mutagenesis are shown. B. Luciferase reporter assay illustrating direct binding of miR-15a to the WT, but not Mut sequences within the 3’-UTR of TNFAIP1. C. mRNA levels of TNFAIP1 were determined by real-time PCR in transfected MG-63 cells. D. Western blots were used to confirm the expression of TNFAIP1 in MG-63 cells after transfection; GAPDH was used as a control. The statistical analysis was based on three independent experiments. Error bars represent mean ± SD. *P < 0.05 vs. untreated or scramble, #P < 0.05 vs. untreated or scramble.