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. 2004 Jul;15(7):3333–3344. doi: 10.1091/mbc.E03-12-0878

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Copyright © 2004, The American Society for Cell Biology

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Figure 3. — GFP-Nup43 and GFP₃-Sec13 coimmunoprecipitate with myc₆-Nup37 and the other constituents of the Nup107-160 complex. Immunoprecipitation using anti-GFP antibodies of soluble extracts from HeLa cells coexpressing GFP-Nup35, GFP-Nup43, or GFP₃-Sec13, and myc₆-Nup37. Equivalent amounts of total extracts (T) and immune supernatants (S) and tenfold equivalents of the immune pellets (P) were analyzed by immunoblot by using anti-GFP, anti-myc (that recognizes the myc₆-Nup37 fusion), Nup85, Nup160, Nup133, Nup96, Nup107, Nup85, or Sec31 antibodies. Arrowheads indicate the position of the GFP fusions. Molecular mass markers on the left are in kilodlatons.