Figure 5.

Constituents of the Nup107-160 complex, but not GFP-Nup35, are targeted to kinetochores. Widefield microscopy images of live interphase and mitotic HeLa cells expressing, as indicated, GFP-Nup85, GFP-Nup35, GFP-Nup160, GFP-Seh1, GFP-Nup37, or GFP-Nup43. Whereas all these GFP-tagged nucleoporins are targeted to the nuclear envelope of interphase cells, fluorescent foci can be visualized at the metaphase plate of mitotic cells expressing these various fusions, with the exception of GFP-Nup35-expressing cells.