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. 2015 Jun 1;8(6):6617–6626.

Figure 2.

Figure 2

miR-218 targets 3’ UTR of HMGB1 and suppresses the expression of HMGB1. (A, B) Luciferase reporter assay with co-transfection of wild-type or mutant HMGB1 and miR-218 mimics or miR-218 inhibitor or mimics-control or inhibitor-control or blank control in RL95-2 (A) and ISK (B) cells. Error bars represent ± S.E. and *, P < 0.05 versus control. (C, D) Western blot analysis was performed to examine the effects of Tax-treat on expression of HMGB1. HMGB1 is up-regulated in Tax-treated endometrial carcinoma cells. (E) qRT-PCR analyses were performed to determine the mRNA levels of HMGB1. (F) The decreased expression of HMGB1 in miR-218 over expressing cells.