Figure 3.

HMGB1 regulates autophagy during chemotherapy in osteosarcoma cells. A-C. Tax treatment promoted the conversion of LC3B-I to LC3B-II and the Beclin1 expression and assayed by Western blot. β-action was detected as the loading control, bars represent mean ± S.E. from three independent experiments (**P < 0.05 vs. control, where n = 3). D and E. HMGB1 gene knocked out in RL95-2 cells and Ishikawa cells. Western blot results showed the expression of HMGB1 protein in endometrial carcinoma cells. F-H. HMGB1 silencing affected the expression of LC3B and the Beclin1 in RL95-2 cells and Ishikawa cells. In control group, RL95-2 cells and Ishikawa cells were treated with Tax. In Talen group, RL95-2 cells and Ishikawa cells were HMGB1 gene knock-out cells and treated with Tax.