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. 2015 Jun 1;8(6):6646–6655.

Figure 2.

Figure 2

miR-1228 promoted breast cancer cell proliferation and reduces cellular apoptosis. A and B: The efficiency of miR-1228 and anti-miR-1228 were detected with RT-PCR in MCF-7 and MDA-MB-468 cells, respectively. C and D: Cell viability was determined for 12 h, 24 h, 36 h, and 48 h using MTT assay with MCF-7 cells transfected with miR-1228 or MDA-MB-468 transfected with anti-miR-1228. E and F: Cells long-term proliferation capacity was determined by colony formation assay with MCF-7 cells transfected with miR-1228 or MDA-MB-468 cells transfected with anti-miR-1228. G and H: The cells apoptosis was detected using Annexin V assay with MCF-7 cells transfected with miR-1228 or MDA-MB-468 cells transfected anti-miR-1228. I: Western blot was performed to detect the effect of miR-1228 and anti-miR-1228 on the expression of MOAP1 in MCF-7 and MDA-MB-468 cells, respectively.