Figure 3.

Ctf13p activation is required to interact with Ndc10p. (A) Cell lysates with the indicated recombinant proteins were prepared as described above (Figure 1), combined with recombinant Cep3p and Ndc10p and analyzed in a bandshift assay. (B) Glutathione Sepharose was incubated with the lysates containing the indicated recombinant proteins. Bound proteins were eluted and immunoblotted. Input lanes represent the levels of protein before isolation. The slower migration of Skp1-4p is due to the addition of a MRGS-His10-HA amino-terminal epitope tag. (C) Levels of GST-Ctf13p or GST bound Cep3p or Ndc10p were equalized to the levels of GST fusion in the binding assay and plotted.