Table 1.
Plasmids used
| Plasmid | Description | Primer pair | Source or reference |
|---|---|---|---|
| pHPR131 | ADH2pr-PTS2-DsRed | Stein et al. (2002) | |
| pKat61 | PEX19 in pPC86/SalI-SacI | RE171/172 | This study |
| pKat79 | PEX19 in pGex4T-2/BamHI-SalI | RE103/104 | This study |
| pPC86-PEX14 | PEX14 in pPC86 | Albertini et al. (1997) | |
| pKat31 | PEX13 (1-151) in pPC97/SmaI-SpeI | RE25/32 | This study |
| pKat32 | PEX13 (1-166) in pPC97/SmaI-SpeI | RE25/33 | This study |
| pKat33 | PEX13 (1-264) in pPC97/SmaI-SpeI | RE25/34 | This study |
| pKat38 | PEX13 (1-386) in pPC97/SmaI-SpeI | RE25/38 | This study |
| pKat39 | PEX13 (151-386) in pPC97/SmaI-SpeI | RE26/38 | This study |
| pKat40 | PEX13 (166-386) in pPC97/SmaI-SpeI | RE27/38 | This study |
| pKat42 | PEX13 (280-386) in pPC97/SmaI-SpeI | RE29/38 | This study |
| pKat143 | PEX13 (173-233) in pPC97/SalI-NotI | RE560/561 | This study |
| pKat144 | PEX13 (221-310) in pPC97/SalI-NotI | RE562/563 | This study |
| pKat145 | PEX13 (173-258) in pPC97/SalI-NotI | RE560/564 | This study |
| pHPR281 | PEX13 (1-386) L207A in pPC97 | RE25/38 | This study |
| pHPR229 | PEX13 (1-386) L207P in pPC97 | RE25/38 | This study |
| pHPR221 | PEX13 (173-258) L207A in pPC97 | RE560/564 | This study |
| pHPR262 | PEX13 (173-258) L207P in pPC97 | RE560/564 | This study |
| pKat71 | PEX11 (1-236) in pPC97/SalI-SacI | RE167/168 | Rottensteiner et al. (2003) |
| pHPR274 | PEX11 (1-236) L35G in pPC97 | RE167/168 | This study |
| pHPR275 | PEX11 (1-236) L35P in pPC97 | RE167/168 | This study |
| pHPR231 | PEX11 (1-50) in pPC97/SalI-SacI | RE700/701 | This study |
| pHPR236 | PEX11 (51-236) in pPC97/SalI-SacI | RE702/168 | This study |
| pPC97/45 | PEX25 (1-394) in pPC97/BglII-SalI | RE57/55 | This study |
| pPC97/63 | PEX25 (147-394) in pPC97/BglII-SalI | RE63/55 | This study |
| pPC97/62 | PEX25 (1-136) in pPC97/BglII-SacI | RE57/62 | This study |
| pMS9 | PEX13 (1-386) in pUG35/BamHI-EcoRI | RE432/435 | Stein et al. (2002) |
| pMS15 | PEX13 (166-264) in pUG35/BamHI-EcoRI | RE434/437 | This study |
| pMS16 | PEX13 (151-264) in pUG35/BamHI-EcoRI | RE433/437 | This study |
| pMS22 | PEX13 (166-310) in pUG35/BamHI-EcoRI | RE434/568 | This study |
| pHPR227 | PEX13 (166-310) L207A in pUG35 | RE434/568 | This study |
| pHPR228 | PEX13 (166-310) L207P in pUG35 | RE434/568 | This study |
| pHPR241 | PEX13 (200-310) in pUG35/BamHI-EcoRI | RE703/568 | This study |
| pMS21 | PEX13 (221-310) in pUG35/BamHI-EcoRI | RE567/568 | This study |
| pHPR300 | PEX13 (200-216) in pUG35/BamHI-HindIII | RE872/873 | This study |
| pHPR293 | PEX13 (200-264) in pUG35/BamHI-EcoRI | RE703/437 | This study |
| pHPR252 | PEX11 (28-40)-PEX13 (213-310) in pUG35 | RE704/568 | This study |