Table 1.
Quantification of Golgi-derived tubule formation in neurons overexpressing wt-LIMK1, LIMK1-kd, and Δ-LIM
| Groups | Numbera | Length (μm) | Duration (sec)b |
|---|---|---|---|
| Control | 2.6 ± 0.2 | 4.2 ± 0.4 | 18 ± 6 |
| wt-LIMK1 | 0.02 ± 0.01c | — | — |
| LIMK1-kd | 2.2 ± 0.2 | 8.5 ± 0.6* | 45 ± 8 |
| Δ-LIM | 2.5 ± 0.2 | 3.8 ± 0.6 | 21 ± 4 |
a
Number of tubules emerging from the Golgi stacks per minute.
b
Duration represents the period of time elapsed between the formation of a new tubule and its retraction and disappearance into the main Golgi stacks.
c
Values significantly different from those of control cells or cells contransfected with Gal-T2-EYFP (2 μg DNA) plus Δ-LIM (4 μg DNA). Each value represents the mean ± SEM. Seventy transfected cells were analyzed for each experimental condition. Each value is the mean ± S.E.M.