Table 2.
Quantification of cofilin, phospho-cofilin, and phalloidin fluorescence intensity in neurons overexpressing wt-LIMK1, LIMK1-kd, and Δ-LIM
| Anti-cofilin (1 μg/ml) | Golgi region | Growth cones |
|---|---|---|
| Non transfected | 1250 ± 65 | 1640 ± 20 |
| wt-LIMK1 | 1225 ± 58 | 1750 ± 56 |
| LIMK1-kd | 1356 ± 46 | 1630 ± 48 |
| Δ-LIM | 1278 ± 84 | 1670 ± 38 |
| Anti-phospho cofilin (0.5 μg/ml) | Golgi region | Growth cones |
| Nontransfected | 458 ± 72 | 680 ± 26 |
| wt-LIMK1 | 1852 ± 64a | 2240 ± 44a |
| Δ-LIM | 450 ± 24 | 2720 ± 28a |
| Anti-phospho cofilin (1 μg/ml) | Golgi region | Growth cones |
| Nontransfected | 932 ± 68 | 1125 ± 55 |
| LIMK1-kd | 184 ± 15a | 272 ± 14a |
| Rhodamine-phalloidin (0.2 μg/ml) | Golgi region | Growth cones |
| Nontransfected | 249 ± 17 | 660 ± 24 |
| wt-LIMK1 | 1246 ± 68a | 1850 ± 48a |
| Δ-LIM | 312 ± 32 | 2412 ± 18a |
| Rhodamine-phalloidin (0.5 μg/ml) | Golgi region | Growth cones |
| Nontransfected | 383 ± 47 | 1256 ± 22 |
| LIMK1-kd | 52 ± 12a | 325 ± 18a |
| S3A-Cofilin | 65 ± 14a | 420 ± 36a |
Values represent the average fluorescent intensity expressed in pixels within a 5-μm area located in the central region of the Golgi apparatus or growth cones. 12-Bit images were used for this quantification. Scale, 0 (black) to 4095 (white). Each value is the mean ± SEM. At least 50 cells were measured for each experimental condition.
a
Values significantly different from those of nontransfected cells.