. 2004 Jul;15(7):3433–3449. doi: 10.1091/mbc.E03-05-0328

Table 4.

Quantification of Par3 immunofluorescence in neurons overexpressing LIMK1 or its mutants

Groups	Antibody dilution	Cell body	Distal axon	Growth cone
NT	1/200	1320 ± 46	125 ± 18^a	181 ± 23
NT	1/50	1854 ± 66	263 ± 52	560 ± 74
WT	1/200	1050 ± 28	340 ± 25^a	650 ± 64^a
KD	1/200	1710 ± 92	60 ± 32	110 ± 15^a
KD	1/50	2050 ± 124	140 ± 14^a	315 ± 17^a
Δ-LIM^b	1/200	2023 ± 87	136 ± 22	176 ± 30
WT + S3A	1/200	1415 ± 39	158 ± 16^a	240 ± 34
LIM^c	1/50	1950 ± 65	134 ± 16	205 ± 15^a
Δ-PDZ^d	1/200	1268 ± 18	186 ± 14	480 ± 26^a

Values represent the average fluorescent intensity expressed in pixels within a 5 μm area located in the cell body, distal axonal segment, and growth cones. 12-Bit images were used for this quantification. Scale, 0 (black) to 4095 (white). Each value is the mean ± S.E.M. Cultures were fixed and processed for immunofluorescence 18 h after transfection. At least 50 cells were analyzed for each experimental condition.

Values significantly different from those of the corresponding control groups.

This group corresponds to cells transfected with the Δ-LIM mutant of wt-LIMK1.

This group corresponds to cell transfected with the LIM domain alone.

This group corresponds cells transfected with the Δ-PDZ mutant of wt-LIMK1.