. 2004 Jul;15(7):3433–3449. doi: 10.1091/mbc.E03-05-0328

Table 5.

Quantification of synaptophysin-GFP and NCAM-GFP immunofluorescence in neurons overexpressing wt-LIMK1 and its mutants

Group	Golgi region	Proximal neurites	Distal neurites	Growth cones
P38-GFP
Control	1450 ± 40	542 ± 38	640 ± 56	850 ± 65
wt-LIMK1	1750 ± 20	160 ± 22^a	156 ± 24^a	132 ± 16^a
LIMK1-kd	1500 ± 35	656 ± 34	720 ± 62	1350 ± 60^a
Δ-LIM	1400 ± 30	528 ± 44	615 ± 27	920 ± 25
NCAM-GFP
Control	1500 ± 65	364 ± 41	488 ± 46	1250 ± 80
wt-LIMK1	1300 ± 45	725 ± 74^a	742 ± 38^a	1880 ± 56^a
LIMK1-kd	1780 ± 20	156 ± 32^a	131 ± 43^a	148 ± 28^a
Δ-PDZ	1430 ± 30	652 ± 64^a	728 ± 40^a	1640 ± 60^a

Control cells were transfected with 2 μg/ml of synaptophysin-GFP or NCAM-GFP cDNAs. Values represent the average fluorescent intensity expressed in pixels within a 5-μm area located in the central region of the Golgi apparatus or growth cones. 12-Bit images were used for this quantification. Scale, 0 (black) to 4095 (white). Values represent synaptophysin-GFP or NCAM-GFP fluorescence intensities within a 30 μm stretch located in the proximal or distal segments of individual neurites. Each value is the mean ± SEM. At least 50 cells were analyzed for each experimental condition.

Values significantly different from those of the corresponding control groups.