Table 2.
Time course (hours) | Condition | Genes | |||
---|---|---|---|---|---|
| |||||
CYCS | Casp3 | Casp8 | Casp9 | ||
0 | Normoxia | 1.00 (0.58~1.73) | 1.00 (0.83~1.20) | 1.00 (0.83~1.22) | 1.00 (0.82~1.22) |
Hypoxia | 0.22 (0.17~0.27) | 0.15 (0.12~0.17) | 0.33 (0.27~0.40) | 0.03 (0.03~0.04)* | |
8 | Normoxia | 0.36 (0.31~0.42) | 0.29 (0.25~0.34) | 0.35 (0.28~0.44) | 0.22 (0.19~0.26) |
Hypoxia | 0.20 (0.17~0.23) | 0.46 (0.39~0.54) | 0.21 (0.18~0.24) | 0.03 (0.03~0.04) | |
24 | Normoxia | 0.38 (0.31~0.46) | 1.12 (0.95~1.31) | 0.32 (0.27~0.37) | 0.62 (0.53~0.74) |
Hypoxia | 0.41 (0.36~0.47) | 6.15 (4.08~9.26)* | 4.43 (3.92~5.00)* | 0.63 (0.52~0.76) | |
72 | Normoxia | 0.25 (0.18~0.36) | 0.29 (0.25~0.34) | 0.31 (0.26~0.38) | 0.10 (0.08~0.13) |
Hypoxia | 1.02 (0.86~1.21) | 2.47 (2.14~2.85)* | 3.10 (2.52~3.81)* | 1.42 (1.16~1.75)* |
The genes expression levels were calcaulted by the 2-ΔΔCT method. Data were presented as 2-ΔΔCT and range.
Indicated significance (p < 0.05) when compared with normoxia or hypoxia.
CYCS, Cytochrome C, Somatic; Casp, caspase.