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. 2004 Jul;15(7):3464–3474. doi: 10.1091/mbc.E03-10-0753

Figure 10.

Figure 10.

Analysis of EGF receptor dimerization in the presence or absence of TM peptide by using a cross-linking agent. A431 cells were transfected or not with the pSecTag plasmid encoding for the EGFR TM peptide. At day 2 after transfection, cells were stimulated or not with EGF (10-8 M, 5 min), cross-linked with disuccinimidyl suberate, and lysed. Samples were immunoprecipitated and submitted to SDS-PAGE electrophoresis and Western blotting with anti-EGF receptor antibodies. (A) Typical blot out of four experiments. The first two columns represent cells transfected with the EGFR TM peptide without (-) and with (+) stimulation with EGF. Columns 3 and 4 represent control untransfected cells. (B) Mean ratio (± SEM) of dimer versus total receptor (dimer/monomer + dimer) in EGF-stimulated cells after densitometric analysis of the blots.