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Results of PCR analysis. Presence of Streptococcus pneumoniae is shown in the lung and the adrenal gland of the patient by PCR analyses. Specific targets were amplified by real-time PCR using the ply and the lytA gene from S. pneumonia and β-actin as an internal control, showing detection of the 225-, the 272-, and the 150-bp products, respectively. M; marker, C1; control case 1, C2; control case 2.
