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GFP-MLC expressed in NIH 3T3 fibroblasts or gerbil fibroma cells incorporates into stress fibers. (A) NIH 3T3 or (C) gerbil fibroma cells were transiently transfected with GFP-MLC and double-labeled with fluorescently tagged phalloidin to show filamentous actin (B and D). (E) Center-to-center spacing (periodicity) of GFP-MLC in NIH 3T3 cells decreased in the periphery of stress fibers after stimulation of contractility, whereas the periodicity in the central regions increased.
