Table 4.
Propagation of CTN-1V or PM virus in the Walvax-2 or MRC-5 cells
| Virus Passage NO. | CTN-1V virus ( log FFU/ml)a | PVvirus ( log FFU/ml)a | ||||
|---|---|---|---|---|---|---|
| Walvax-2 cells | MRC-5 cells | Pb | Walvax-2 cells | MRC-5 cells | Pb | |
| original | 7.50 | 7.50 | / | 7.50 | 7.50 | / |
| 1 | 4.84± 0.62 | 4.58± 0.40 | >0.05 | 4.40± 0.27 | 3.62± 0.23 | >0.05 |
| 2 | 5.40± 0.21 | 5.02±0.34 | <0.05 | 5.04±0.18 | 4.75± 0.24 | <0.05 |
| 3 | 6.10± 0.37 | 5.41± 0.24 | <0.05 | 5.30± 0.33 | 4.83± 0.25 | <0.05 |
| 4 | 6.530.31 | 6.09± 0.17 | <0.05 | 5.86± 0.10 | 5.02± 0.13 | <0.05 |
| 5 | 6.78± 0.40 | 6.14± 0.16 | <0.05 | 6.21± 0.21 | 5.63± 0.05 | <0.05 |
| 6 | 7.08± 0.15 | 6.57± 0.42 | >0.05 | 6.57± 0.53 | 6.02± 0.18 | >0.05 |
| 7 | 7.34± 0.22 | 6.89± 0.21 | <0.05 | 7.01± 0.70 | 6.00± 0.23 | >0.05 |
| 8 | 7.51± 0.21 | 7.16± 0.08 | >0.05 | 6.93± 0.19 | 6.28± 0.25 | <0.05 |
| 9 | 7.67± 0.18 | 7.09± 0.10 | <0.05 | 7.23± 0.23 | 6.59± 0.26 | <0.05 |
| 10 | 8.14± 0.31 | 7.41± 0.35 | <0.05 | 8.02± 0.19 | 7.11± 0.38 | <0.05 |
Passages the 1th to 4th, subculture; Passages the 5th to 8th, cell-mixing; Passages the 9th to 10th, cell-free medium;
a±SD.
b Significance of difference (P value) determined by 2-tailed t-test