Table I.

Primer sequences used in reverse transcription polymerase chain reaction.

Gene	Primer sequence	Tm (°C)	Fragment size (bp)	Cycle
CD29	F: 5′-GAAACTTGGTGGCATCGT-3′
	R: 5′-CTCAGTGAAGCCCAGAGG-3′	60	493	30
CD44	F: 5′-CGGAACATAGGGTTTGAGA-3′
	R: 5′-GGTTGATGTCTTCTGGGTTA-3′	56	301	30
CD73	F: 5′-CAATGGCACGATTACCTG-3′
	R: 5′-GACCTTCAACTGCTGGATA-3′	60	426	30
CD166	F: 5′-TATCAGGATGCTGGAAAC-3′
	R: 5′-TAGCCAATAGACGACACC-3′	55	498	30
CD34	F: 5′-CCTCATCAGCTTTGCGACTT-3′
	R: 5′-CCAGGAGCAAGGAGCACA-3′	60	454	30
CD45	F: 5′-CTACCCAACCTTCTACTCAA-3′
	R: 5′-TTCACATCCAGGAGGTTC-3′	56	221	30
BOLA-DRα	F: 5′-AGCACTTGGGTTTGAATG-3′
	R: 5′-GGAGCAGTAGACAGGGAAT-3′	58	404	30
COLLA2	F: 5′-AGAAGCATGTCTGGGTAGGAG-3′
	R: 5′-AGGATAGGCAGGCGAGATR-3′	59	358	30
OPN	F: 5′-ATACCCTCCCAAGTAAGTC-3′
	R: 5′-TGTGATGTGAAGTCCTCC-3′	56	340	30
MAP-2	F: 5′-GAGAACGGAATCAACGGAGAAC-3′
	R: 5′-CCAAACAGAGTGGGAGGTGC-3′	60	396	30
Nestin	F: 5′-AGAGGAGAACGCTGAGTCATT-3′
	R: 5′-TCTGTAGGCTTTAGTGGTTCTG-3′	54	537	30
GAPDH	F: 5′-GGCAAGTTCAACGGCACAGTCA-3′
	R: 5′-TAAGTCCCTCCACGATGCCAAAG-3′	60	365	30

Tm, temperature, F, forward; R, reverse; MAP-2, microtubule-associated protein 2; OPN, osteopontin; COLLA2, collagen type I α2; BOLA, bovine lymphocyte Ag.