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Primers used for reverse transcription quantitative polymerase chain reaction.
| Gene | Primer sequence (5′–3′) | Dissociation temperature (°C) | Product (bp) |
|---|---|---|---|
| β-actin | F-CCTGTACGCCAACACAGTGC R-ATACTCCTGCTTGCTGATCC |
60 | 211 |
| IL-1Ra (NM_031167.5) |
F-ACCAAATATCAAACTAGAAGAAAA R-CAGAGCGGATGAAGGTAA |
56 | 200 |
F, forward; R, reverse; IL-1Ra, interleukin-1 receptor antagonist.
