Figure 5.

Inhibition of invasion by overexpression of NPRL2. Transwell assays were performed to analyze the effects of the overexpression of NPRL2 on the invasive potential of colorectal cancer cells. Attached cells in the lower section were stained with hematoxylin and eosin and counted using light microscopy (magnification, x200). Control, cells without transfection; mock, cells transfected with mock vector; NPRL2, cells transfected with NPRL2-overexpression vector. NPRL2, nitrogen permease regulator-like-2; GFP, green fluorescent protein.