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SAN inhibits reactive oxygen species generation induced by Ang II. The climbing glasses of cells were collected and mounted using SlowFade Gold antifade reagent with DAPI. Changes in the density of dichlorodihydrofluorescein diacetate fluorescence (green) were confirmed by fluorescence microscopy (magnification, x200). (A) Control; (B) Ang II (10 µM); (C) Ang II (10 µM) + SAN 0.5 µM; (D) Ang II (10 µM) + N-acetylcysteine (1 mM). Ang, angiotensin; SAN, sanguinarine.
