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. 2015 May 25;12(3):3400–3408. doi: 10.3892/mmr.2015.3841

Figure 5.

Figure 5

Effects of SAN on MMP loss induced by Ang II. (A) H9c2 cardiac cells were pre-treated with various concentrations (0.125, 0.25 and 0.5 µM) of SAN for 40 min prior to being co-treated with Ang II (10 µM) for 24 h. JC-1 staining indicated that in the Ang II group, the amount of monomers was increased, while that of multimers was decreased compared with that in the control group. When H9c2 cardiac cells were co-treated with the indicated concentrations of SAN, the decreased MMP returned to normal and (a) the number of JC-1 multimers was increased, while (b) monomers were decreased. (B) Treatment of the cells with NAC (1 mM) and Ang II (10 µM) for 24 h caused similar changes in the MMP changes to that following treatment with SAN, with (a) increases in JC-1 multimers and (d) decreases in monomers. Values are expressed as the mean ± standard error of the mean for three independent experiments. #P<0.01 vs. control; *P<0.01 vs. Ang II group. SAN, sanguinarine; Ang, angiotensin; ROS, reactive oxygen species; NAC, N-acetylcysteine; MMP, mitochondrial membrane potential.