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. 2015 Jun 3;12(3):3359–3364. doi: 10.3892/mmr.2015.3888

Figure 2.

Figure 2

Figure 2

Ectopic expression of miR-199b-5p inhibits cell proliferation, inhibits the cell cycle at the G1- to S-phase transition, induces cell apoptosis and suppresses cell invasion in ES cells. (A) Expression of miR-199b-5p significantly inhibited the cell proliferation in A673/TC252 cells. (B) Flow cytometry was performed to assess the cell cycle and revealed that miR-199b-5p arrested cells at the G1- to S-phase transition. (C) An apoptosis assay demonstrated that miR-199b-5p induced cell apoptosis. In the dot plots, top left quadrant : dead cells; bottom left quadrant: living cells; bottom right quadrant: cells in early apoptosis; and top right quadrant: cells in late apoptosis. (D) An invasion assay revealed that miR-199b-5p suppressed the cell invasion ability. miR, microRNA.