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. 2015 Jun 3;12(3):3359–3364. doi: 10.3892/mmr.2015.3888

Figure 3.

Figure 3

CCNL1 is the target gene of miR-199b-5p in vitro. (A) The nucleotide sequences of miR-199b-5p and the complementary sequence in CCNL1 mRNAs revealed the potential binding site. (B) The luciferase activity was performed in the transfected 293T cells and demonstrated a significant decrease in the mRNA expression levels of CCNL1. The miR-199b-5p mimic significantly suppressed the luciferase activity of the reporter gene, containing CCNL1-WT, however, not CCNL1-MUT. The data are expressed as the mean ± standard deviation of three independent experiments (*P<0.05, **P<0.01, compared with the scramble control). miR, microRNA; WT, wild-type; MT, mutant.