Figure 3.

Zinc finger E-box-binding homeobox 2 (ZEB2) is the target of microRNA (miR)-141. (A) Schematic diagram of the putative miR-141-binding sites in the ZEB2 3′-untranslated region (UTR) region, as detected by TargetScan. ZEB2-mut indicates the ZEB2 3′-UTR with mutation in miR-141-binding sites. (B and C) Regulation of luciferase activity by ZEB2 3′-UTR is dependent on miR-141. (B) HGC-27 and (C) SGC-7901 human gastric cancer cells were transfected with the pRL-TK containing a Renilla luciferase gene, and the indicated vectors or precursors. Bars indicate the Firefly luciferase activities normalized to Renilla luciferase activities of the cotransfected pRL-TKvector. Each experiment was repeated at least three times, and each sample was assayed in triplicate. Data is expressed as the mean ± standard error from three independent experiments. ^*P<0.05, vs. Negative.