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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Neurobiol Dis. 2015 Mar 31;78:172–195. doi: 10.1016/j.nbd.2015.02.031

Fig. 1. Generation and expression analysis in G2019S KI mice.

Fig. 1

(a) Schematic of targeting design, showing the G2019S mutation in exon 41. The PKG-Neo-pA cassette was removed by Cre-mediated deletion. (b) Confirmation, via cDNA sequencing, of the presence of the two base mutagenesis which was required to change the codon that encodes amino acid 2019 from GGG to AGC in exon 41. (c) Expression analysis of striatal cDNA via quantitative RT-PCR for genes LRRK1, LRRK2, MAPT and SNCA did not reveal any differences between genotypes. Data expressed as mean ± SEM. (d) Protein expression in the hemi-brain (detected by LRRK2 antibody MJFF2) was similar at 3 months between genotypes. (e) Protein levels of LRRK2 and TH in the striatum at 3, 12 and 20 months. GAPDH is the loading control.