. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: Clin Exp Pharmacol Physiol. 2014 Oct;41(10):780–787. doi: 10.1111/1440-1681.12285

Table 1.

Whole-animal cholesterol content in Npc1^+/+ and Npc1^−/− mice started on 2-hydroxypropyl-β-cyclodextrin treatment in early adulthood

Genotype	Treatment starting at 49 days of age	Age at time of study (days)	Whole-animal cholesterol content (mg/kg bw)
Npc1^+/+	——	49	2240±51^a
Npc1^−/−	——	49	5016±137^b
Npc1^+/+	Saline	77	2212±45^a
Npc1^−/−	Saline	77	6865±161^c
Npc1^+/+	2HPβCD	77	2229±48^a
Npc1^−/−	2HPβCD	77	3977±213^d

Values are mean±1 SEM of data from 7 animals in each group (3 males and 4 females in every case). The 77-day old mice received four successive subcutaneous injections of either saline or 2HPβCD weekly, starting at 49 days of age. These data are from the same mice used for whole-organ cholesterol content measurements and the plasma ALT and AST activity (Figures 2 and 4, respectively). Different letters (a–d) denote statistically different values (P<0.05) as determined by 2-way ANOVA with genotype, treatment and age as variables.