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. 2015 Aug 5;9(8):e0003924. doi: 10.1371/journal.pntd.0003924

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© 2015 Kumar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A. Adherence of ETEC H10407 to HCT-8 cells in the presence of antisera. The fold-change in ETEC H10407 adherence is plotted as a function of incubation with the indicated antisera, after normalization to control samples. Asterisks indicate the antisera showing significant protection against ETEC H10407 that were selected for further characterization, n = 3/group. B. Analysis of antisera specificity. Deletion mutants were constructed for the skp, mipA, and ETEC_2479 genes and then analyzed for their adherence to HCT-8 cells in the presence of the indicated antisera. Asterisks indicate significant reduction in bacterial adherence, n = 4-12/group. C. Heat map displaying the fold-change in adherence of the indicated ETEC strains to HCT-8 cells after their incubation with the indicated antisera. The scale ranges from 0.5 to a 10-fold decrease in adherence, as compared with control samples, n = 3/group. Red colors depict reduced ETEC adherence in the presence of antisera, while green colors depict increased adherence. The midpoint of the color gradient was set as a 2-fold reduction in adherence (black).