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. 2015 Aug 5;10(8):e0133991. doi: 10.1371/journal.pone.0133991

Fig 4. Hyalomin-1 does not inhibit or interact with γ-thrombin.

Fig 4

(A) Steady state kinetic analysis of γ-thrombin in the absence (filled circles) or presence (filled squares) of 600 nM hyalomin-1. (B) γ-Thrombin (black circles) and α-thrombin (black squares)-catalyzed hydrolysis S2238 (50 μM) in the presence of hyalomin-1. (C) Binding of γ-thrombin and α-thrombin to immobilized hyalomin-1 measured by SPR. Buffer alone (1), 50 nM γ-thrombin (2), 100 nM γ-thrombin (3), 50 nM α-thrombin (4), 100 nM α-thrombin (5).