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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Cell Metab. 2015 Jun 25;22(2):253–265. doi: 10.1016/j.cmet.2015.05.022

Figure 7. Simvastatin Increases BDNF in the Brain of FAD5X Mice via PPARα.

Figure 7

(A–E) Genotyping of FAD5X/Ppara null (5X/αKO) mice. Five-month-old mice were fed simvastatin for 2 weeks followed by immunofluorescence analysis of astroglial BDNF (GFAP, red; BDNF, green) in the cortices of (B) wild-type (WT), (C) αKO, (D) FAD5X, and (E) FAD5X/αKO animals.

(F and G) Immunoblot (F) and densitometric (G) analyses of CREB and BDNF were performed in the hippocampal extracts.

(H) ELISA of BDNF in the hippocampal extracts.

(I and J) Number of errors (I) and latency (J) in Barnes maze for statin unfed and fed WT, αKO, FAD5X, and FAD5X/αKO.

(K–N) All groups of mice were also monitored for body weight (K), stereotypy (L), total distance (M), and horizontal activity (N). Eight mice (n = 8) were used in each group. *p < 0.05 versus statin-unfed wild-type control and **p < 0.05 versus statin-unfed FAD5X mice.